Journal: The Journal of Experimental Medicine

Article Title: The XC chemokine receptor 1 is a conserved selective marker of mammalian cells homologous to mouse CD8α + dendritic cells

doi: 10.1084/jem.20100223

Figure Lengend Snippet: XCR1 is functionally active on mouse CD8α + , human BDCA3 + , and sheep CD26 + DCs, and Xcl1 mRNA is stored in quiescent NK cells and memory CD8 + T lymphocytes. Transwell migration assay on enriched human blood DCs or lymphocytes, sheep lymph CD26 + versus CD26 − DCs, and splenic DCs from XCR1 −/− and C57BL/6NCrl (XCR1 +/+ ) mice. Results are representative of at least two independent experiments for each species and expressed as mean ± SEM from duplicate wells for each data point. (B) Expression of the XCL1 gene in human and mouse cell types and tissues, based on the same gene chips data as used in , with the following additions: black triangles, NK cells; purple triangles, resting peripheral CD8 + T cells; purple diamond, anti-CD3 activated human T cells; purple square, mouse CD8 + thymocytes; violet square, mouse CD4 + thymocytes. Results are expressed as mean and SD for at least three independent values for most human data points. (C) Expression of the XCL1 gene in sheep leukocytes as assessed by real-time PCR on the same lymph or blood cells as shown in . Results are mean ± SEM of triplicate real-time RT-PCR reactions, and they are representative of two different sheep for lymphocytes and of three different sheep for DCs. (D) Results of Xcl1 gene expression in mouse CD8 + T cell subsets. Xcl1 expression was measured by real-time PCR on sorted naive or T IM , or antiviral T CM CD8 + T cell subsets. Expression of Ccl5 and Ifng were also evaluated as controls, as the genes are expressed to higher levels in memory CD8 + T cells . Results are represented as mean ± SD for mean values from duplicate real-time PCR reactions performed on mRNAs from naive T cells or from T IM from three individual mice each, and from T CM from four individual pools of seven mice.

Article Snippet: The lower chamber was filled with migration medium alone or containing 300 ng/ml of mouse recombinant XCL1 (R&D Systems) for mouse cells, 500 ng/ml of human recombinant XCL1 (R&D Systems) for human cells, and 100 ng/ml of recombinant mouse XCL1 for sheep cells.

Techniques: Transwell Migration Assay, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression

Journal: eLife

Article Title: Cytotoxic T cells swarm by homotypic chemokine signalling

doi: 10.7554/eLife.56554

Figure Lengend Snippet:

Article Snippet: Peptide, recombinant protein , Recombinant Mouse XCL1 , R & D systems , Cat. #: 486-LT-025 , (1, 10 and 100 ng/ml).

Techniques: In Vivo, Cell Culture, Transduction, Construct, Sequencing, Produced, Transfection, Expressing, Plasmid Preparation, Clone Assay, Control, Recombinant, In Vitro, Mass Spectrometry, Cell Isolation, Selection, Reverse Transcription, Sandwich ELISA, Cytometry, Software